| Jordan Anthony | XCEL Athletics Track & Field Club | 20.52 | qualified | declared |
| Connor Washington | University of Arkansas, Fayetteville | 20.60 | qualified | declared |
| Laurenz Colbert | Baylor University | 20.64 | qualified | declared |
| Maximilian Thomas | Evo Track Club | 20.68 | qualified | declared |
| Anthony Greenhow Jr. | University of South Carolina, Columbia | 20.78 | qualified | declared |
| Kashie Crockett | University of Louisiana at Lafayette | 20.79 | qualified | declared |
| Brandon Miller | | 20.79 | qualified | declared |
| Michael Gizzi | University of Alabama in Huntsville | 20.91 | qualified | declared |
| Gavin Schurr | | 20.92 | qualified | declared |
| Jaylen Slade | adidas | 21.02 | qualified | declared |
| Koryee Wyatt Jr. | Oklahoma State University | 21.16 | qualified | declared |
| Alexander Nunley | North Carolina State University | 21.17 | qualified | declared |
| Christian Trapps | Cheetah Elite | 21.19 | qualified | declared |
| Terrell Robinson Jr. | | 21.32 | qualified | declared |
| Aidan McDuffie | | 21.35 | qualified | declared |
| Derrick Harbin | | 21.37 | qualified | declared |
| Xavier Parris | Kennesaw State University | 21.40 | qualified | declared |
| Tyson Tippett | | 21.32 | not qualified | not accepted |
| Miles Stephens | Appalachian State University | 21.57 | not qualified | not accepted |
| Jaiden Reed | | 21.35 | not qualified | scratched |
| Dom Dutton | University of South Carolina, Columbia | 21.41 | not qualified | scratched |
| Nyckoles Harbor II | | 20.79 | qualified | |
| Judson Lincoln IV | Bullseye Running | 21.25 | qualified | |
| Iyana Gray | University of Texas at Arlington | 22.78 | qualified | declared |
| Jayla Jamsion | University of South Carolina, Columbia | 23.02 | qualified | declared |
| Shawnti Jackson | | 23.06 | qualified | declared |
| Kaniya Johnson | Fast Forward Track Club | 23.20 | qualified | declared |
| Mia Brahe-Pedersen | The Inner Circle Track Club | 23.23 | qualified | declared |
| Tierra Frasier | University of South Carolina, Columbia | 23.30 | qualified | declared |
| Kennedi Sanders | XCEL Athletics Track & Field Club | 23.51 | qualified | declared |
| Madison Whyte | | 23.57 | qualified | declared |
| Tiffani Rae Pittman | Howard University | 23.58 | qualified | declared |
| Autumn Wilson | | 23.68 | qualified | declared |
| Camryn Dickson | | 23.70 | qualified | declared |
| Dajaz Defrand | Malone Panthers Track Club | 23.74 | qualified | declared |
| Lily Jones | | 23.75 | qualified | declared |
| Summer Sergeant | | 23.78 | qualified | declared |
| BreeAna Bates | University of Michigan | 23.86 | qualified | declared |
| Michaela Francois | Baylor University | 23.88 | qualified | declared |
| Raykiyat Olukoju | | 23.89 | qualified | declared |
| Alyssa Colbert | Northern Arizona University | 24.12 | qualified | declared |
| Mirai Bernard | | 24.14 | qualified | declared |
| Anivea Gilbert | Sam Houston State University | 24.14 | qualified | declared |
| Anya Jackson | Northwest Flyers Track Club | 24.23 | qualified | declared |
| Janiyah Fields | University of North Carolina, Wilmington | 24.35 | qualified | declared |
| Kaila Jackson | | 23.01 | accepted | declared |
| Brianna Clayton | University of South Carolina Upstate | 24.41 | not qualified | not accepted |
| Yahnari Lyons | Drive Phase Track Club | 22.99 | qualified | scratched |
| Emily Ervin | College of William and Mary | 23.92 | qualified | scratched |
| Caleia Johnson | | 24.19 | qualified | |
| Jasmine Montgomery | University of Oregon | 23.12 | rejected | |
| Morgan Smith | | 26.58 | not qualified |
