| Men 400m | ||||
|---|---|---|---|---|
| Name | Affiliation | Mark | Status | Declaration |
| Steven McElroy II | University of Cincinnati | 45.57 | qualified | declared |
| Charlie Bartholomew Jr. | Oklahoma State University | 45.94 | qualified | declared |
| Ashton Schwartzman | Texas A&M University, College Station | 45.98 | qualified | declared |
| William Jones | KC Flyers Track Club | 46.29 | qualified | declared |
| Darius Rainey | The Wings Track Club | 46.36 | qualified | declared |
| Brandon Battle | University of Arkansas, Fayetteville | 46.37 | qualified | declared |
| Caemon Scott | University of Louisiana at Lafayette | 46.38 | qualified | declared |
| Andrew Brown | 46.66 | qualified | declared | |
| Avaunt Ortiz | Phoenix Elitez | 46.69 | qualified | declared |
| Carl Hicks Jr. | University of Arizona | 47.06 | qualified | declared |
| Devin Clark | Cobb PAL | 47.31 | qualified | declared |
| Sydney Peal | Union Athletics | 47.55 | qualified | declared |
| Will Sumner | 45.78 | qualified | scratched | |
| Judson Lincoln IV | Bullseye Running | 46.37 | qualified | |
| Lyricc Lopez | GLS Speed TC | 46.65 | qualified | |
| Cameron Mullens | 51.60 | not qualified | ||
| Women 400m | ||||
| Name | Affiliation | Mark | Status | Declaration |
| Mekenze Kelley | Coastal Carolina University | 51.98 | qualified | declared |
| Kennedy Wade | Texas A&M University, College Station | 52.48 | qualified | declared |
| Kennedi Sanders | XCEL Athletics Track & Field Club | 52.62 | qualified | declared |
| Victoria Bossong | Harvard University | 53.01 | qualified | declared |
| Haley Tate | 53.15 | qualified | declared | |
| Hali Murphy | 53.23 | qualified | declared | |
| Kaylyn Brown | Purpose Driven NC Elite Track Club | 53.30 | qualified | declared |
| Zaya Akins | 53.41 | qualified | declared | |
| Charlee Crawford | Rutgers, The State University of New Jersey, | 54.48 | qualified | declared |
| Sage Hinton | 54.50 | qualified | declared | |
| Brilee Pontius | Brigham Young University | 54.63 | qualified | declared |
| Emily Ervin | College of William and Mary | 54.69 | qualified | declared |
| Madison Whyte | 54.71 | qualified | declared | |
| Mirai Bernard | 54.76 | qualified | declared | |
| Sophia Gorriaran | Providence Cobras | 54.10 | qualified | scratched |
| Caleia Johnson | 55.03 | qualified | scratched | |
