| Jordan Anthony | XCEL Athletics Track & Field Club | 10.14 | qualified | declared |
| Michael Gizzi | University of Alabama in Huntsville | 10.27 | qualified | declared |
| Terrell Robinson Jr. | | 10.31 | qualified | declared |
| Laurenz Colbert | Baylor University | 10.33 | qualified | declared |
| Gavin Schurr | | 10.37 | qualified | declared |
| Derrick Harbin | | 10.40 | qualified | declared |
| Maximilian Thomas | Evo Track Club | 10.40 | qualified | declared |
| Kashie Crockett | University of Louisiana at Lafayette | 10.41 | qualified | declared |
| Brandon Miller | | 10.41 | qualified | declared |
| Connor Washington | University of Arkansas, Fayetteville | 10.41 | qualified | declared |
| David Foster | University of California, Berkeley | 10.42 | qualified | declared |
| Christian Trapps | Cheetah Elite | 10.43 | qualified | declared |
| Jaiden Reed | | 10.45 | qualified | declared |
| Aidan McDuffie | | 10.51 | qualified | declared |
| Elijah Major | University of Detroit Mercy | 10.57 | qualified | declared |
| Alexander Nunley | North Carolina State University | 10.59 | qualified | declared |
| Jaylen Slade | adidas | 10.59 | qualified | declared |
| Tyson Tippett | | 10.60 | qualified | declared |
| Anthony Greenhow Jr. | University of South Carolina, Columbia | 10.63 | not qualified | not accepted |
| Miles Stephens | Appalachian State University | 10.68 | not qualified | not accepted |
| Xavier Parris | Kennesaw State University | 10.69 | not qualified | not accepted |
| Dom Dutton | University of South Carolina, Columbia | 10.57 | qualified | scratched |
| Nyckoles Harbor II | | 10.28 | qualified | |
| Jaden Clark | Ferrum College | 10.58 | not qualified | |
| Iyana Gray | University of Texas at Arlington | 11.22 | qualified | declared |
| Jayla Jamsion | University of South Carolina, Columbia | 11.26 | qualified | declared |
| Kaniya Johnson | Fast Forward Track Club | 11.26 | qualified | declared |
| Alyssa Colbert | Northern Arizona University | 11.33 | qualified | declared |
| Mia Brahe-Pedersen | The Inner Circle Track Club | 11.34 | qualified | declared |
| Autumn Wilson | | 11.36 | qualified | declared |
| Victoria Perrow | | 11.37 | qualified | declared |
| Lily Jones | | 11.44 | qualified | declared |
| Shawnti Jackson | | 11.48 | qualified | declared |
| BreeAna Bates | University of Michigan | 11.54 | qualified | declared |
| Michaela Francois | Baylor University | 11.58 | qualified | declared |
| Summer Sergeant | | 11.60 | qualified | declared |
| Tierra Frasier | University of South Carolina, Columbia | 11.61 | qualified | declared |
| Tiffani Rae Pittman | Howard University | 11.61 | qualified | declared |
| Alyssa Tate | | 11.62 | qualified | declared |
| Dajaz Defrand | Malone Panthers Track Club | 11.64 | qualified | declared |
| Anivea Gilbert | Sam Houston State University | 11.70 | qualified | declared |
| Camryn Dickson | | 11.84 | qualified | declared |
| Simone Ballard | | 11.86 | qualified | declared |
| Kaila Jackson | | 11.51 | accepted | declared |
| Raykiyat Olukoju | | 11.93 | not qualified | not accepted |
| Anya Jackson | Northwest Flyers Track Club | 12.01 | not qualified | not accepted |
| Yahnari Lyons | Drive Phase Track Club | 11.40 | qualified | scratched |
| Payton Payne | | 11.86 | qualified | |
| Caleia Johnson | | 11.88 | qualified | |
| Jasmine Montgomery | University of Oregon | 11.35 | rejected | |
| Eleyah Armstrong | Virginia Military Institute | 11.83 | rejected | |
| Jade Ofotan | Glenarden Track Club | 11.89 | not qualified | |
| Takiya Henson | | 11.95 | not qualified | |
| Morgan Smith | | 12.74 | not qualified |
