| Team | Seed |
| Born To Run | 3:00 |
| Jacobs Sports Academy | 3.57 |
| West Dallas Wings Elite Track | 3.58 |
| Major Impact Texas | - |
| Houston Westside | 25.00h |
| Elite Sports Performance | 3:42.00h |
| Athletic Performance Ranch | 3:44.00h |
| H-Town Hurricanes | 3:44.00h |
| Athletic Performance Ranch | 3:46.00h |
| San Antonio Swift Track Club | 3:47.00h |
| Fort Bend Marshall | 3:47.01 |
| Steadfast Track Academy | 3:48.00 |
| AMAX Speed | 3:49.00h |
| Fort Bend Christian Academy | 3:49.43 |
| REAL Training | 3:49.50 |
| Wings Track Club | 3:50.00 |
| APXP Speed Development | 3:50.42 |
| Athletic Performance Ranch | 3:52.00h |
| Athletic Performance Ranch | 3:53.00h |
| Xtreme Force Track Club | 3:53.21 |
| Athletic Performance Ranch | 3:54.00h |
| Lake Charles Express | 3:54.30 |
| Track Houston Youth Track Club | 3:55.00 |
| College Station Sprinters | 3:55.00h |
| APXP Speed Development | 3:55.01 |
| Katy Mayde Creek | 3:55.26 |
| Afterburners Youth Track Club | 3:55.28 |
| H-Town Hurricanes | 3:55.91 |
| LC Express | 3:56.00 |
| Magnolia | 3:56.21 |
| Steadfast Track Academy | 3:57.00 |
| Wings Track Club | 3:58.00 |
| Killeen Chaparral | 3:58.00h |
| Track Dynamite | 3:58.00h |
| Team All-Stars | 3:58.11 |
| Houston Visions Track Club | 3:59.00 |
| REAL Training | 3:59.50 |
| Houston Cyp. Fair | 4:00.00h |
| Houston St Agnes | 4:00.00h |
| Iowa Colony | 4:00.00h |
| Killeen Harker Heights | 4:00.00h |
| Westside Baptist Church TC | 4:00.00h |
| Wings Track Club | 4:01.00 |
| Dallas Carter | 4:02.00h |
| Richmond Elite YTC | 4:03.01 |
| Texas Select | 4:04.00h |
| Ferris | 4:04.82 |
| Team All-Stars | 4:05.00 |
| Bryan Rudder | 4:05.00h |
| Houston Westbury | 4:05.00h |
| West | 4:09.00h |
| Dallas Carter | 4:10.00h |
| Fort Bend Marshall | 4:10.00h |
| Clear Brook | 4:15.00h |
| Xtreme Force Track Club | 4:15.45 |
| Houston Westbury | 4:20.00h |
| Born To Run | 4:20.53 |
| Kingsville King | 4:31.05 |
| Palestine | 4:44.00h |
